Review





Similar Products

apc  (Miltenyi Biotec)
97
Miltenyi Biotec apc
Apc, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/apc/product/Miltenyi Biotec
Average 97 stars, based on 1 article reviews
apc - by Bioz Stars, 2026-02
97/100 stars
  Buy from Supplier
anti human kv1 3  (Alomone Labs)
93
Alomone Labs anti human kv1 3
Protein and mRNA expression of typical ion channels in THP-1 cells. Monocytic THP-1 cells have been stimulated with PMA overnight (under exactly the same conditions as for the nanostraw experiments). After that, cells were incubated under normoxia (24 h; NOX) or under hypoxic conditions (HOX: 1% O 2 ) for 2 h, 4 h, and 24 h respectively. ( a ) mRNA expression of TRPM2 , TRPM4 and KCNA3 (encoding <t>Kv1.3)</t> under the given conditions. Whereas TRPM2 showed a significant downregulation after 24 h of hypoxic incubation, TRPM4 mRNA was upregulated. KCNA3 mRNA showed no changes (mean ± SD; n = 9; one way ANOVA plus Dunnett´s multiple comparisons test; ***: p < 0.001, ****: p < 0.0001). ( b ) TRPM4 and Kv1.3 protein are expressed in THP-1 cells under normoxic conditions and can be found in the cytosol and membrane of the cells (400x magnification, scale bar: 20 μm) but did not show altered expression under hypoxia (data not shown). ( c ) TRPM2 protein was cleaved under hypoxic conditions and shorter versions of the protein became detectable after only 2 h of hypoxia. 50 µg of whole cell lysate have been applied to Western blotting. Blot is representative for three independent results. ( d ) Intracellular protein distribution analysis of TRPM2 showed a prominent overall downregulation of TRPM2 after 2 h of hypoxia especially in areas close to the cell membrane. This effect seemed to recover over time (400x magnification, scale bar: 20 μm). Negative control (without primary antibody) did not show staining for TRPM2 at all
Anti Human Kv1 3, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti human kv1 3/product/Alomone Labs
Average 93 stars, based on 1 article reviews
anti human kv1 3 - by Bioz Stars, 2026-02
93/100 stars
  Buy from Supplier
staining with anti cd303 bdca 2 apc  (Miltenyi Biotec)
95
Miltenyi Biotec staining with anti cd303 bdca 2 apc
Protein and mRNA expression of typical ion channels in THP-1 cells. Monocytic THP-1 cells have been stimulated with PMA overnight (under exactly the same conditions as for the nanostraw experiments). After that, cells were incubated under normoxia (24 h; NOX) or under hypoxic conditions (HOX: 1% O 2 ) for 2 h, 4 h, and 24 h respectively. ( a ) mRNA expression of TRPM2 , TRPM4 and KCNA3 (encoding <t>Kv1.3)</t> under the given conditions. Whereas TRPM2 showed a significant downregulation after 24 h of hypoxic incubation, TRPM4 mRNA was upregulated. KCNA3 mRNA showed no changes (mean ± SD; n = 9; one way ANOVA plus Dunnett´s multiple comparisons test; ***: p < 0.001, ****: p < 0.0001). ( b ) TRPM4 and Kv1.3 protein are expressed in THP-1 cells under normoxic conditions and can be found in the cytosol and membrane of the cells (400x magnification, scale bar: 20 μm) but did not show altered expression under hypoxia (data not shown). ( c ) TRPM2 protein was cleaved under hypoxic conditions and shorter versions of the protein became detectable after only 2 h of hypoxia. 50 µg of whole cell lysate have been applied to Western blotting. Blot is representative for three independent results. ( d ) Intracellular protein distribution analysis of TRPM2 showed a prominent overall downregulation of TRPM2 after 2 h of hypoxia especially in areas close to the cell membrane. This effect seemed to recover over time (400x magnification, scale bar: 20 μm). Negative control (without primary antibody) did not show staining for TRPM2 at all
Staining With Anti Cd303 Bdca 2 Apc, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/staining with anti cd303 bdca 2 apc/product/Miltenyi Biotec
Average 95 stars, based on 1 article reviews
staining with anti cd303 bdca 2 apc - by Bioz Stars, 2026-02
95/100 stars
  Buy from Supplier
cd87 apc  (Miltenyi Biotec)
91
Miltenyi Biotec cd87 apc
Protein and mRNA expression of typical ion channels in THP-1 cells. Monocytic THP-1 cells have been stimulated with PMA overnight (under exactly the same conditions as for the nanostraw experiments). After that, cells were incubated under normoxia (24 h; NOX) or under hypoxic conditions (HOX: 1% O 2 ) for 2 h, 4 h, and 24 h respectively. ( a ) mRNA expression of TRPM2 , TRPM4 and KCNA3 (encoding <t>Kv1.3)</t> under the given conditions. Whereas TRPM2 showed a significant downregulation after 24 h of hypoxic incubation, TRPM4 mRNA was upregulated. KCNA3 mRNA showed no changes (mean ± SD; n = 9; one way ANOVA plus Dunnett´s multiple comparisons test; ***: p < 0.001, ****: p < 0.0001). ( b ) TRPM4 and Kv1.3 protein are expressed in THP-1 cells under normoxic conditions and can be found in the cytosol and membrane of the cells (400x magnification, scale bar: 20 μm) but did not show altered expression under hypoxia (data not shown). ( c ) TRPM2 protein was cleaved under hypoxic conditions and shorter versions of the protein became detectable after only 2 h of hypoxia. 50 µg of whole cell lysate have been applied to Western blotting. Blot is representative for three independent results. ( d ) Intracellular protein distribution analysis of TRPM2 showed a prominent overall downregulation of TRPM2 after 2 h of hypoxia especially in areas close to the cell membrane. This effect seemed to recover over time (400x magnification, scale bar: 20 μm). Negative control (without primary antibody) did not show staining for TRPM2 at all
Cd87 Apc, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd87 apc/product/Miltenyi Biotec
Average 91 stars, based on 1 article reviews
cd87 apc - by Bioz Stars, 2026-02
91/100 stars
  Buy from Supplier
cd44 apc  (Miltenyi Biotec)
96
Miltenyi Biotec cd44 apc
Protein and mRNA expression of typical ion channels in THP-1 cells. Monocytic THP-1 cells have been stimulated with PMA overnight (under exactly the same conditions as for the nanostraw experiments). After that, cells were incubated under normoxia (24 h; NOX) or under hypoxic conditions (HOX: 1% O 2 ) for 2 h, 4 h, and 24 h respectively. ( a ) mRNA expression of TRPM2 , TRPM4 and KCNA3 (encoding <t>Kv1.3)</t> under the given conditions. Whereas TRPM2 showed a significant downregulation after 24 h of hypoxic incubation, TRPM4 mRNA was upregulated. KCNA3 mRNA showed no changes (mean ± SD; n = 9; one way ANOVA plus Dunnett´s multiple comparisons test; ***: p < 0.001, ****: p < 0.0001). ( b ) TRPM4 and Kv1.3 protein are expressed in THP-1 cells under normoxic conditions and can be found in the cytosol and membrane of the cells (400x magnification, scale bar: 20 μm) but did not show altered expression under hypoxia (data not shown). ( c ) TRPM2 protein was cleaved under hypoxic conditions and shorter versions of the protein became detectable after only 2 h of hypoxia. 50 µg of whole cell lysate have been applied to Western blotting. Blot is representative for three independent results. ( d ) Intracellular protein distribution analysis of TRPM2 showed a prominent overall downregulation of TRPM2 after 2 h of hypoxia especially in areas close to the cell membrane. This effect seemed to recover over time (400x magnification, scale bar: 20 μm). Negative control (without primary antibody) did not show staining for TRPM2 at all
Cd44 Apc, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd44 apc/product/Miltenyi Biotec
Average 96 stars, based on 1 article reviews
cd44 apc - by Bioz Stars, 2026-02
96/100 stars
  Buy from Supplier
cd325 apc  (Miltenyi Biotec)
94
Miltenyi Biotec cd325 apc
Protein and mRNA expression of typical ion channels in THP-1 cells. Monocytic THP-1 cells have been stimulated with PMA overnight (under exactly the same conditions as for the nanostraw experiments). After that, cells were incubated under normoxia (24 h; NOX) or under hypoxic conditions (HOX: 1% O 2 ) for 2 h, 4 h, and 24 h respectively. ( a ) mRNA expression of TRPM2 , TRPM4 and KCNA3 (encoding <t>Kv1.3)</t> under the given conditions. Whereas TRPM2 showed a significant downregulation after 24 h of hypoxic incubation, TRPM4 mRNA was upregulated. KCNA3 mRNA showed no changes (mean ± SD; n = 9; one way ANOVA plus Dunnett´s multiple comparisons test; ***: p < 0.001, ****: p < 0.0001). ( b ) TRPM4 and Kv1.3 protein are expressed in THP-1 cells under normoxic conditions and can be found in the cytosol and membrane of the cells (400x magnification, scale bar: 20 μm) but did not show altered expression under hypoxia (data not shown). ( c ) TRPM2 protein was cleaved under hypoxic conditions and shorter versions of the protein became detectable after only 2 h of hypoxia. 50 µg of whole cell lysate have been applied to Western blotting. Blot is representative for three independent results. ( d ) Intracellular protein distribution analysis of TRPM2 showed a prominent overall downregulation of TRPM2 after 2 h of hypoxia especially in areas close to the cell membrane. This effect seemed to recover over time (400x magnification, scale bar: 20 μm). Negative control (without primary antibody) did not show staining for TRPM2 at all
Cd325 Apc, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd325 apc/product/Miltenyi Biotec
Average 94 stars, based on 1 article reviews
cd325 apc - by Bioz Stars, 2026-02
94/100 stars
  Buy from Supplier
cd324 apc  (Miltenyi Biotec)
93
Miltenyi Biotec cd324 apc
Protein and mRNA expression of typical ion channels in THP-1 cells. Monocytic THP-1 cells have been stimulated with PMA overnight (under exactly the same conditions as for the nanostraw experiments). After that, cells were incubated under normoxia (24 h; NOX) or under hypoxic conditions (HOX: 1% O 2 ) for 2 h, 4 h, and 24 h respectively. ( a ) mRNA expression of TRPM2 , TRPM4 and KCNA3 (encoding <t>Kv1.3)</t> under the given conditions. Whereas TRPM2 showed a significant downregulation after 24 h of hypoxic incubation, TRPM4 mRNA was upregulated. KCNA3 mRNA showed no changes (mean ± SD; n = 9; one way ANOVA plus Dunnett´s multiple comparisons test; ***: p < 0.001, ****: p < 0.0001). ( b ) TRPM4 and Kv1.3 protein are expressed in THP-1 cells under normoxic conditions and can be found in the cytosol and membrane of the cells (400x magnification, scale bar: 20 μm) but did not show altered expression under hypoxia (data not shown). ( c ) TRPM2 protein was cleaved under hypoxic conditions and shorter versions of the protein became detectable after only 2 h of hypoxia. 50 µg of whole cell lysate have been applied to Western blotting. Blot is representative for three independent results. ( d ) Intracellular protein distribution analysis of TRPM2 showed a prominent overall downregulation of TRPM2 after 2 h of hypoxia especially in areas close to the cell membrane. This effect seemed to recover over time (400x magnification, scale bar: 20 μm). Negative control (without primary antibody) did not show staining for TRPM2 at all
Cd324 Apc, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd324 apc/product/Miltenyi Biotec
Average 93 stars, based on 1 article reviews
cd324 apc - by Bioz Stars, 2026-02
93/100 stars
  Buy from Supplier
anti glast acsa 1 apc antibody  (Miltenyi Biotec)
97
Miltenyi Biotec anti glast acsa 1 apc antibody
Protein and mRNA expression of typical ion channels in THP-1 cells. Monocytic THP-1 cells have been stimulated with PMA overnight (under exactly the same conditions as for the nanostraw experiments). After that, cells were incubated under normoxia (24 h; NOX) or under hypoxic conditions (HOX: 1% O 2 ) for 2 h, 4 h, and 24 h respectively. ( a ) mRNA expression of TRPM2 , TRPM4 and KCNA3 (encoding <t>Kv1.3)</t> under the given conditions. Whereas TRPM2 showed a significant downregulation after 24 h of hypoxic incubation, TRPM4 mRNA was upregulated. KCNA3 mRNA showed no changes (mean ± SD; n = 9; one way ANOVA plus Dunnett´s multiple comparisons test; ***: p < 0.001, ****: p < 0.0001). ( b ) TRPM4 and Kv1.3 protein are expressed in THP-1 cells under normoxic conditions and can be found in the cytosol and membrane of the cells (400x magnification, scale bar: 20 μm) but did not show altered expression under hypoxia (data not shown). ( c ) TRPM2 protein was cleaved under hypoxic conditions and shorter versions of the protein became detectable after only 2 h of hypoxia. 50 µg of whole cell lysate have been applied to Western blotting. Blot is representative for three independent results. ( d ) Intracellular protein distribution analysis of TRPM2 showed a prominent overall downregulation of TRPM2 after 2 h of hypoxia especially in areas close to the cell membrane. This effect seemed to recover over time (400x magnification, scale bar: 20 μm). Negative control (without primary antibody) did not show staining for TRPM2 at all
Anti Glast Acsa 1 Apc Antibody, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti glast acsa 1 apc antibody/product/Miltenyi Biotec
Average 97 stars, based on 1 article reviews
anti glast acsa 1 apc antibody - by Bioz Stars, 2026-02
97/100 stars
  Buy from Supplier
antibody solution apc ctnt  (Miltenyi Biotec)
95
Miltenyi Biotec antibody solution apc ctnt
Protein and mRNA expression of typical ion channels in THP-1 cells. Monocytic THP-1 cells have been stimulated with PMA overnight (under exactly the same conditions as for the nanostraw experiments). After that, cells were incubated under normoxia (24 h; NOX) or under hypoxic conditions (HOX: 1% O 2 ) for 2 h, 4 h, and 24 h respectively. ( a ) mRNA expression of TRPM2 , TRPM4 and KCNA3 (encoding <t>Kv1.3)</t> under the given conditions. Whereas TRPM2 showed a significant downregulation after 24 h of hypoxic incubation, TRPM4 mRNA was upregulated. KCNA3 mRNA showed no changes (mean ± SD; n = 9; one way ANOVA plus Dunnett´s multiple comparisons test; ***: p < 0.001, ****: p < 0.0001). ( b ) TRPM4 and Kv1.3 protein are expressed in THP-1 cells under normoxic conditions and can be found in the cytosol and membrane of the cells (400x magnification, scale bar: 20 μm) but did not show altered expression under hypoxia (data not shown). ( c ) TRPM2 protein was cleaved under hypoxic conditions and shorter versions of the protein became detectable after only 2 h of hypoxia. 50 µg of whole cell lysate have been applied to Western blotting. Blot is representative for three independent results. ( d ) Intracellular protein distribution analysis of TRPM2 showed a prominent overall downregulation of TRPM2 after 2 h of hypoxia especially in areas close to the cell membrane. This effect seemed to recover over time (400x magnification, scale bar: 20 μm). Negative control (without primary antibody) did not show staining for TRPM2 at all
Antibody Solution Apc Ctnt, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/antibody solution apc ctnt/product/Miltenyi Biotec
Average 95 stars, based on 1 article reviews
antibody solution apc ctnt - by Bioz Stars, 2026-02
95/100 stars
  Buy from Supplier

Image Search Results


Protein and mRNA expression of typical ion channels in THP-1 cells. Monocytic THP-1 cells have been stimulated with PMA overnight (under exactly the same conditions as for the nanostraw experiments). After that, cells were incubated under normoxia (24 h; NOX) or under hypoxic conditions (HOX: 1% O 2 ) for 2 h, 4 h, and 24 h respectively. ( a ) mRNA expression of TRPM2 , TRPM4 and KCNA3 (encoding Kv1.3) under the given conditions. Whereas TRPM2 showed a significant downregulation after 24 h of hypoxic incubation, TRPM4 mRNA was upregulated. KCNA3 mRNA showed no changes (mean ± SD; n = 9; one way ANOVA plus Dunnett´s multiple comparisons test; ***: p < 0.001, ****: p < 0.0001). ( b ) TRPM4 and Kv1.3 protein are expressed in THP-1 cells under normoxic conditions and can be found in the cytosol and membrane of the cells (400x magnification, scale bar: 20 μm) but did not show altered expression under hypoxia (data not shown). ( c ) TRPM2 protein was cleaved under hypoxic conditions and shorter versions of the protein became detectable after only 2 h of hypoxia. 50 µg of whole cell lysate have been applied to Western blotting. Blot is representative for three independent results. ( d ) Intracellular protein distribution analysis of TRPM2 showed a prominent overall downregulation of TRPM2 after 2 h of hypoxia especially in areas close to the cell membrane. This effect seemed to recover over time (400x magnification, scale bar: 20 μm). Negative control (without primary antibody) did not show staining for TRPM2 at all

Journal: Pflugers Archiv

Article Title: Just a little prick: careful cell contacts enabled by ceramic nanostraws

doi: 10.1007/s00424-026-03150-7

Figure Lengend Snippet: Protein and mRNA expression of typical ion channels in THP-1 cells. Monocytic THP-1 cells have been stimulated with PMA overnight (under exactly the same conditions as for the nanostraw experiments). After that, cells were incubated under normoxia (24 h; NOX) or under hypoxic conditions (HOX: 1% O 2 ) for 2 h, 4 h, and 24 h respectively. ( a ) mRNA expression of TRPM2 , TRPM4 and KCNA3 (encoding Kv1.3) under the given conditions. Whereas TRPM2 showed a significant downregulation after 24 h of hypoxic incubation, TRPM4 mRNA was upregulated. KCNA3 mRNA showed no changes (mean ± SD; n = 9; one way ANOVA plus Dunnett´s multiple comparisons test; ***: p < 0.001, ****: p < 0.0001). ( b ) TRPM4 and Kv1.3 protein are expressed in THP-1 cells under normoxic conditions and can be found in the cytosol and membrane of the cells (400x magnification, scale bar: 20 μm) but did not show altered expression under hypoxia (data not shown). ( c ) TRPM2 protein was cleaved under hypoxic conditions and shorter versions of the protein became detectable after only 2 h of hypoxia. 50 µg of whole cell lysate have been applied to Western blotting. Blot is representative for three independent results. ( d ) Intracellular protein distribution analysis of TRPM2 showed a prominent overall downregulation of TRPM2 after 2 h of hypoxia especially in areas close to the cell membrane. This effect seemed to recover over time (400x magnification, scale bar: 20 μm). Negative control (without primary antibody) did not show staining for TRPM2 at all

Article Snippet: The first antibody (anti-human TRPM2 from rabbit, RRID: AB_1716520, VWR International GmbH, Darmstadt, Germany, #ABNOPAB11990), anti-human TRPM4 from rabbit, (VWR International GmbH, Darmstadt, Germany, #BOSSBS-9051R, RRID: AB_3712801) or anti-human Kv1.3 from rabbit (#APC-101, RRID: AB_2040149, Alomone labs, Jerusalem, Israel) was added at a concentration of 1:100 in 3% BSA-PBS for 2 h. Cells were washed with PBS before the second antibody (goat anti-rabbit Alexa Fluor488 (#A-11008; RRID: AB_143165) for TRPM2 and goat anti-rabbit Alexa Fluor568 (#A-11011, RRID: AB_143157) for TRPM4 and Kv1.3, both from Life Technologies, Darmstadt, Germany and used in a dilution of 1:400 in PBS) was added for additional 90 min. Cover glasses were fixed with fluorescence mounting medium (#S3023, Agilent, Santa Clara, United States of America) on the slides, and cells were analyzed the following day with a fluorescence microscope (AxioVert with Axiocam 305; Zeiss, Oberkochen, Germany).

Techniques: Expressing, Incubation, Membrane, Western Blot, Negative Control, Staining